SidaGhanaV1, Main, Exploration, bibRecord, 001367

Detection of circulating human immunodeficiency virus type 2 in plasma by reverse transcription polymerase chain reaction

Identifieur interne : 001367 ( Main/Exploration ); précédent : 001366; suivant : 001368

Detection of circulating human immunodeficiency virus type 2 in plasma by reverse transcription polymerase chain reaction

Auteurs : I. Loussert-Ajaka [France] ; F. Simon [France] ; I. Farfara [France] ; D. Descamps [France] ; G. Collin [France] ; F. Brun-Vézinet [France]

Source :

Research in Virology [ 0923-2516 ] ; 1995.

RBID : ISTEX:1E663DC9CC143156444AD025C1A0C5254B71AC95

English descriptors

Abstract

Summary: Genomic RNA was detected using a reverse transcription (RT) nested polymerase chain reaction (PCR) method on plasma from 24 HIV2-infected patients. Results were interpreted based on immune and clinical status and results of plasma and cellular viraemia assays. Amplification of RNA extracted from plasma was positive in 13 of the 24 cases studied (54%). There was a negative correlation between the detection of RNA in plasma and the patients' CD4+ cell counts: all 5 patients with counts below 200×106/l were RT-PCR RNA-positive, compared to only 4 of the 12 patients with counts above 500×106/l. Cellular viraemia was positive in 12 of the 24 patients, and the results correlated with the CD4+ cell count. HIV2 was isolated from the plasma of 3 of the 24 patients, all of whom had CD4+ cell counts below 200×106/l. The small viral load in HIV2-infected patients before the onset of immunodeficiency appeared to be a major limiting factor in the detection of the virus with current tests. The low percentage of RNA-positive plasma samples contrasts with the high rate of positivity in HIV1-infected patients. Differences in viral load and replication between HIV1 and HIV2 correlate with differences in the epidemiology and pathogenicity of the two viruses.

Url:

https://api.istex.fr/document/1E663DC9CC143156444AD025C1A0C5254B71AC95/fulltext/pdf

DOI: 10.1016/0923-2516(96)80900-9

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 000693
to stream Istex, to step Curation: 000693
to stream Istex, to step Checkpoint: 000818
to stream Main, to step Merge: 001477
to stream Main, to step Curation: 001367

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Detection of circulating human immunodeficiency virus type 2 in plasma by reverse transcription polymerase chain reaction</title>
<author><name sortKey="Loussert Ajaka, I" sort="Loussert Ajaka, I" uniqKey="Loussert Ajaka I" first="I." last="Loussert-Ajaka">I. Loussert-Ajaka</name>
</author>
<author><name sortKey="Simon, F" sort="Simon, F" uniqKey="Simon F" first="F." last="Simon">F. Simon</name>
</author>
<author><name sortKey="Farfara, I" sort="Farfara, I" uniqKey="Farfara I" first="I." last="Farfara">I. Farfara</name>
</author>
<author><name sortKey="Descamps, D" sort="Descamps, D" uniqKey="Descamps D" first="D." last="Descamps">D. Descamps</name>
</author>
<author><name sortKey="Collin, G" sort="Collin, G" uniqKey="Collin G" first="G." last="Collin">G. Collin</name>
</author>
<author><name sortKey="Brun Vezinet, F" sort="Brun Vezinet, F" uniqKey="Brun Vezinet F" first="F." last="Brun-Vézinet">F. Brun-Vézinet</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:1E663DC9CC143156444AD025C1A0C5254B71AC95</idno>
<date when="1995" year="1995">1995</date>
<idno type="doi">10.1016/0923-2516(96)80900-9</idno>
<idno type="url">https://api.istex.fr/document/1E663DC9CC143156444AD025C1A0C5254B71AC95/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000693</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000693</idno>
<idno type="wicri:Area/Istex/Curation">000693</idno>
<idno type="wicri:Area/Istex/Checkpoint">000818</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000818</idno>
<idno type="wicri:doubleKey">0923-2516:1995:Loussert Ajaka I:detection:of:circulating</idno>
<idno type="wicri:Area/Main/Merge">001477</idno>
<idno type="wicri:Area/Main/Curation">001367</idno>
<idno type="wicri:Area/Main/Exploration">001367</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Detection of circulating human immunodeficiency virus type 2 in plasma by reverse transcription polymerase chain reaction</title>
<author><name sortKey="Loussert Ajaka, I" sort="Loussert Ajaka, I" uniqKey="Loussert Ajaka I" first="I." last="Loussert-Ajaka">I. Loussert-Ajaka</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Virologie, Hôpital Bichat/Claude Bernard, 46, rue Henri Huchard, 75877 Paris</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Simon, F" sort="Simon, F" uniqKey="Simon F" first="F." last="Simon">F. Simon</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Virologie, Hôpital Bichat/Claude Bernard, 46, rue Henri Huchard, 75877 Paris</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Farfara, I" sort="Farfara, I" uniqKey="Farfara I" first="I." last="Farfara">I. Farfara</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Virologie, Hôpital Bichat/Claude Bernard, 46, rue Henri Huchard, 75877 Paris</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Descamps, D" sort="Descamps, D" uniqKey="Descamps D" first="D." last="Descamps">D. Descamps</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Virologie, Hôpital Bichat/Claude Bernard, 46, rue Henri Huchard, 75877 Paris</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Collin, G" sort="Collin, G" uniqKey="Collin G" first="G." last="Collin">G. Collin</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Virologie, Hôpital Bichat/Claude Bernard, 46, rue Henri Huchard, 75877 Paris</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Brun Vezinet, F" sort="Brun Vezinet, F" uniqKey="Brun Vezinet F" first="F." last="Brun-Vézinet">F. Brun-Vézinet</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Virologie, Hôpital Bichat/Claude Bernard, 46, rue Henri Huchard, 75877 Paris</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Research in Virology</title>
<title level="j" type="abbrev">X135</title>
<idno type="ISSN">0923-2516</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1995">1995</date>
<biblScope unit="volume">146</biblScope>
<biblScope unit="issue">6</biblScope>
<biblScope unit="page" from="409">409</biblScope>
<biblScope unit="page" to="414">414</biblScope>
</imprint>
<idno type="ISSN">0923-2516</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0923-2516</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amplification</term>
<term>Amplification cycles</term>
<term>Asymptomatic patients</term>
<term>Cell count</term>
<term>Cell counts</term>
<term>Cellular viraemia</term>
<term>Clinical status</term>
<term>Culture supematants</term>
<term>Culture supernatants</term>
<term>Detection</term>
<term>Detection rates</term>
<term>Fresh plasma</term>
<term>Fresh plasma samples</term>
<term>Genetic diversity</term>
<term>Genetic variability</term>
<term>Geographic distribution</term>
<term>HIV2</term>
<term>Hiv2</term>
<term>Hiv2 infection</term>
<term>Hiv2infected patients</term>
<term>Immunodeficiency</term>
<term>Lymphocyte count</term>
<term>Natural history</term>
<term>PCR</term>
<term>Pbmc</term>
<term>Peripheral blood</term>
<term>Plasma</term>
<term>Plasma viraemia</term>
<term>Polymerase</term>
<term>Polymerase chain reaction</term>
<term>Primer</term>
<term>Primer pair</term>
<term>Primer pairs</term>
<term>RNA</term>
<term>Reverse transcription</term>
<term>Rnase inhibitor</term>
<term>Sujets</term>
<term>Transcription</term>
<term>Vih2</term>
<term>Viraemia</term>
<term>Viral</term>
<term>Viral culture</term>
<term>Viral load</term>
<term>West africa</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Amplification</term>
<term>Amplification cycles</term>
<term>Asymptomatic patients</term>
<term>Cell count</term>
<term>Cell counts</term>
<term>Cellular viraemia</term>
<term>Clinical status</term>
<term>Culture supematants</term>
<term>Culture supernatants</term>
<term>Detection rates</term>
<term>Fresh plasma</term>
<term>Fresh plasma samples</term>
<term>Genetic diversity</term>
<term>Genetic variability</term>
<term>Geographic distribution</term>
<term>Hiv2</term>
<term>Hiv2 infection</term>
<term>Hiv2infected patients</term>
<term>Immunodeficiency</term>
<term>Lymphocyte count</term>
<term>Natural history</term>
<term>Pbmc</term>
<term>Peripheral blood</term>
<term>Plasma</term>
<term>Plasma viraemia</term>
<term>Polymerase</term>
<term>Polymerase chain reaction</term>
<term>Primer</term>
<term>Primer pair</term>
<term>Primer pairs</term>
<term>Rnase inhibitor</term>
<term>Sujets</term>
<term>Transcription</term>
<term>Vih2</term>
<term>Viraemia</term>
<term>Viral</term>
<term>Viral culture</term>
<term>Viral load</term>
<term>West africa</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Summary: Genomic RNA was detected using a reverse transcription (RT) nested polymerase chain reaction (PCR) method on plasma from 24 HIV2-infected patients. Results were interpreted based on immune and clinical status and results of plasma and cellular viraemia assays. Amplification of RNA extracted from plasma was positive in 13 of the 24 cases studied (54%). There was a negative correlation between the detection of RNA in plasma and the patients' CD4+ cell counts: all 5 patients with counts below 200×106/l were RT-PCR RNA-positive, compared to only 4 of the 12 patients with counts above 500×106/l. Cellular viraemia was positive in 12 of the 24 patients, and the results correlated with the CD4+ cell count. HIV2 was isolated from the plasma of 3 of the 24 patients, all of whom had CD4+ cell counts below 200×106/l. The small viral load in HIV2-infected patients before the onset of immunodeficiency appeared to be a major limiting factor in the detection of the virus with current tests. The low percentage of RNA-positive plasma samples contrasts with the high rate of positivity in HIV1-infected patients. Differences in viral load and replication between HIV1 and HIV2 correlate with differences in the epidemiology and pathogenicity of the two viruses.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Île-de-France</li>
</region>
<settlement><li>Paris</li>
</settlement>
</list>
<tree><country name="France"><region name="Île-de-France"><name sortKey="Loussert Ajaka, I" sort="Loussert Ajaka, I" uniqKey="Loussert Ajaka I" first="I." last="Loussert-Ajaka">I. Loussert-Ajaka</name>
</region>
<name sortKey="Brun Vezinet, F" sort="Brun Vezinet, F" uniqKey="Brun Vezinet F" first="F." last="Brun-Vézinet">F. Brun-Vézinet</name>
<name sortKey="Collin, G" sort="Collin, G" uniqKey="Collin G" first="G." last="Collin">G. Collin</name>
<name sortKey="Descamps, D" sort="Descamps, D" uniqKey="Descamps D" first="D." last="Descamps">D. Descamps</name>
<name sortKey="Farfara, I" sort="Farfara, I" uniqKey="Farfara I" first="I." last="Farfara">I. Farfara</name>
<name sortKey="Simon, F" sort="Simon, F" uniqKey="Simon F" first="F." last="Simon">F. Simon</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/SidaGhanaV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001367 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001367 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    SidaGhanaV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:1E663DC9CC143156444AD025C1A0C5254B71AC95
   |texte=   Detection of circulating human immunodeficiency virus type 2 in plasma by reverse transcription polymerase chain reaction
}}

This area was generated with Dilib version V0.6.31.
Data generation: Tue Nov 7 18:07:38 2017. Site generation: Tue Mar 5 15:01:57 2024

	Le SIDA au Ghana (serveur d'exploration)
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Le SIDA au Ghana (serveur d'exploration)

Detection of circulating human immunodeficiency virus type 2 in plasma by reverse transcription polymerase chain reaction

Detection of circulating human immunodeficiency virus type 2 in plasma by reverse transcription polymerase chain reaction

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri